Scandinavian Distributor of Medical Diagnostic and Life Science Products

High Resolution Melting


LCGreen® Plus+ Melting Dye



LCGreen Plus+ is tailored for use in melting instruments with 96- or 384-well microtiter plates. It has superb fluorescence intensity and can be used with other fluorescence based PCR detection systems such as the LightScanner® instrument, but also LightCycler® 480, Rotor-Gene®, CFX96™, and many of Life Tech qPCR systems. For optimal performance, the use of a Hi-Res melting instrument is required.


LCGreen Plus+ Kit Details

Number of Reactions

10x Solution

1 000 Reactions

1 mL

10 000 Reactions

10x 1 mL




Request a FREE sample LCGreen® Plus Melting Dye by emailing info@biotech-igg.com!!


Download information brochure LCGreen Information Sheet.


LightScanner® Mastermix

 

LightScanner® master mix provides a pre-optimized solution specifically designed to deliver superior PCR performance for

Hi-Res Melting® and genotyping applications. This master mix reduces time and saves money with easy-to-use premixed formulas. This secures downstream success by directly going from PCR to melting curve analysis and eliminate the risk of contamination.


LightScanner Master Mix Kit Contents:


This master mix incorporates a Taq polymerase, an antibody-based hot-start method, dNTPs, magnesium chloride, and BioFire Defense 19 proprietary LCGreen® PLUS dye and is formulated at a 2.5X concentration. The antibody-mediated hot-start method means no enzyme pre-activation is required before or during thermocycling, translating into much faster PCR protocol times than other methods of hot-start protection.The master mix includes a concentration of magnesium that is optimized to work for almost all reactions. Additional magnesium chloride solution is provided for further optimization if required. The master mix is also compatible with most additives used to enhance amplification efficiency of high GC amplicons.


LightScanner® Mastermix Kit Contents:


Quantity

Description

1 x 100 Rxn

2.5X Master Mix

1.5 mL

10 mM MgCI2

1.5 mL

Reagent Grade Water

1

LightScanner Master Mix User 19s Guide




Request a FREE sample LightScanner® Mastermix by emailing info@biotech-igg.com!!


Download information brochure Mastermix Information Sheet, and Mastermix Comparison Note.





Product Description Product Code






LCGreen Plus+ Melting Dye (1 000 rxns)
BF-BCHM-ASY-0005
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LCGreen Plus+ Melting Dye (10 000 rxns)
BF-BCHM-ASY-0006
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LightScanner® Mastermix (100 rxns)
BF-HRLS-ASY- 0002
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LightScanner® Mastermix (500 rxns)
BF-HRLS-ASY- 0003
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